Neon Tau Markerlight Drone

Documentation – Arm Developer M·A·C Lipstick – the iconic product that made M·A·C famous. This ultra-creamy formula features a smooth glide, bold colour payoff, full coverage and a semi-lustrous finish. An eclectic collection of fine and faux statement jewelry, often worn by celebrities. Women trust Jennifer Miller to create a personal jewelry style for them that will complete their individual looks, make a statement, and be versatile. Neon EVM - Grow with the best from two Chains. Ethereum Smart Contracts Scaled by Solana

2022.01.25 04:31 OhneSkript Neon Tau Markerlight Drone

Neon Tau Markerlight Drone submitted by OhneSkript to Tau40K [link] [comments]

2022.01.25 04:31 Same-Can9032 [Rhino Prime] [PS4] Any Offers?

[Rhino Prime] [PS4] Any Offers? submitted by Same-Can9032 to wartrade [link] [comments]

2022.01.25 04:31 eokia fun games

how does everyone here have such crazy and funny things going on in their games? i feel like my saves are becoming more and more repetitive despite me playing about 300 hours in the past three years and i set the autonomy/free-will (can’t remember the actual name) to high in hopes it would get better. i even tested it out with some sims multiple times with the high autonomy and the sun life was just so mundane.
submitted by eokia to Sims3 [link] [comments]

2022.01.25 04:31 __Snor1aX__ [H] Falchion Knife | Slaughter FN, Hand Wraps | Arboreal BS [W] Bowie Knife | Marble Fade FN

0.02 fv Falchion,+20$ on
b/o Bowie Knife | Marble Fade FN + YOUR ADDS
submitted by __Snor1aX__ to Csgotrading [link] [comments]

2022.01.25 04:31 Franklo Any tips on lighting this large dining/living room? 8 cans in total. Parents cant settle on a decision.

Any tips on lighting this large dining/living room? 8 cans in total. Parents cant settle on a decision. submitted by Franklo to Lighting [link] [comments]

2022.01.25 04:31 LeadingBiology Common contamination and solutions in cell cultures

Common types of biological pollution in cell culture include bacterial contamination, mold contamination, mycoplasma contamination, black dots contamination, fungal contamination, and protozoa contamination. The characteristics of these contamination in cell culture and the corresponding solutions are as follows:
1. Bacteria contamination
The bacteria present black and fine sand under the ordinary inverted microscope. According to the different kinds of infected bacteria, it may show different shapes, and the culture solution will generally become cloudy and yellow, which has an obvious influence on cell growth.
Solutions: Check the sterilization of utensils carefully to ensure the ventilation time and the pressure of autoclaving is enough. Most of all, items such as pipettes that are in contact with culture medium may cause contamination of the storage solution if it is contaminated twice. So be sure to pay attention! Besides, check the culture medium turbidity before the next use. And, it is recommended to add antibiotics to the culture medium, such as tetracycline, gentamycin, or streptomycin.
2. Mold contamination
Normally, the culture medium is clear and transparent with no impurities under the inverted microscope even if it already has been cultured for two or three days under 37℃ in incubators. Once flocculent impurities occur, filamentous and conglobated floats are microscopically shown and the hyphae is obvious. At this time, although the bacterial cells are still growing, their vital state will gradually deteriorate after a long time.
Solutions: Wipe the CO2 incubator with copper sulfate solution, and add saturated amount of copper sulfate to the tray. Or adding the saturated disodium hydrogen phosphate high salinity solution after disinfection to avoid the mold contamination.
In case of the mold contamination, temporarily transfer all of cells, and immediately and thoroughly use peracetic acid solution to wipe the incubator including its baffle plate and inside wall. Place the peracetic acid in the incubator for an hour to diffuse the steam and transfer the bacteria cells to the incubator after the odor of peracetic acid is dissipated. It's worthwhile to note that the incubator needs regular cleaning every two months, especially in rainy seasons. Wiping incubators with 84 Disinfection Solution, clean water and 75 % alcohol successively and irradiate with ultraviolet light is also a useful method.
In order to prevent the mold contamination, it is necessary to add 3μg/ml amphotericin, mycostatin, actinomycin D or penicillin-streptomycin. Once contaminated, it’s impossible to recover, and it makes no difference to the matter even with aforementioned antibiotics. The best option for mycoplasma-infected cell cultures is to discard infected cultures and replace them with fresh stocks which are mycoplasma-free and disinfect the environment thoroughly. If all the bacteria cells are contaminated, it may be systemic contamination. Therefore, it is necessary to check the culture medium and experiment equipment. If it is only individual contamination, it may be an operational problem. So it is essential to pay attention to the specifications of the experimental operation steps.
3. Mycoplasma contamination
Mycoplasmas are covert contaminants, and it can't be removed by filtration. The absence of any visible signs of mycoplasma contamination, such as cytopathogenicity and changes in turbidity or pH (even in heavily contaminated cultures), leads to a false sense of security. The culture medium usually become turbid after mycoplasma contamination, and interiorly, serums are seldom tested for negative detection of mycoplasma, which is one of the most common microorganisms in bovine serum.
Solutions: Once contaminated by mycoplasma, especially for important cell lines, it is necessary to remove mycoplasma and general methods are antibiotic treatment, antiserum treatment and antibiotics treatment combined with antiserum and alexin. It is noteworthiness that the most prominent structural feature of mycoplasma is the absence of cell walls. Therefore, it is completely insensitive to biosynthetic antibiotics acting on the cell wall, such as β-lactams, vancomycin, etc., and is generally resistant to polymyxin, rifampicin, and sulfa drugs. Instead, tetracyclines and macrolides have the most effective antibiotics inhibitory against mycoplasma, while aminoglycosides and chloramphenicol have less inhibitory effects. Also, all cultures need to be replaced when necessary. Finally, the usual method of filtration sterilization has no effect on mycoplasma.
4. Black dots contamination
The black swimming dots can penetrate the filter membrane and spread through the air. They shows as black dota at low magnification and mobile black specks at high magnification. The culture solution is also not turbid, and generally affects less, so the bacteria cells can still be used. Usually, after black dots contamination, the cells grow well, and the moving objects are not evidently increased, and the color and transparency of the culture medium have no significant change. Such a similar phenomenon can be found in the same batch of serum-cultured cells.
Solutions: Black dots do not have a significant effect on cell growth status, and will naturally disappear after cell proliferation, so no special treatment is needed except for serum replacement. And it is suggested to increase the plate density for the higher survival rate of the cells if they are likely to be contaminated by the small moving dots.
5. Fungi contamination
After fungal contamination, the culture medium is generally clear and remains its original color. Filaments can be observed under the microscope. Initially, some fungi resemble dead cell debris, but many of them are clearly seen, and look like coraline, unlike cell debris indistinguishable. In addition, they will grow thin and black filament slowly because they grow more slower and are not as easily found as bacteria, once the medium are found to be contaminated, they will be difficult to survive.
Solutions: Once contaminated by fungi, discard it decisively, then sterilize the culture room, CO2 incubator, utensils and culture medium thoroughly.
6. Protozoa contamination
After the protozoa contamination, the cells culture medium becomes slightly turbid. Under the microscope, a large number of small dots move back and forth. Although the cells can still grow at this time, the reproduction speed slows down, and the cells have a bad growth state with unclear edges and become non-transparent. Protozoa and bacteria cells form a symbiotic relationship. Simultaneously, protozoa compete with cells for nutrition. This symbiosis is very common, but the cells predominate because the number of protozoa is relatively small, and thus having no effect on the normal growth of cells, only when they reach a certain amount, and eventually become malignant cycle.
Solution: There are many possible causes for protozoa contamination, such as liquid disinfection problems, operational problems, environmental problems, etc. If the cells are enough, discard the contaminated cell decisively and resuscitate cells. If preserved, purchase the relevant sterilization reagents.
submitted by LeadingBiology to RNA [link] [comments]

2022.01.25 04:31 champshitonly96 Who would you use to complete my Real P&P team? I’m set on prime Raul up top, need a RM CB & RB

Who would you use to complete my Real P&P team? I’m set on prime Raul up top, need a RM CB & RB submitted by champshitonly96 to fut [link] [comments]

2022.01.25 04:31 hackintoshuser Volume and Brightness keys do not work while moving windows.

I was listening to music while someone was talking to me so tapped on volume mute key while moving the rhythmbox window but I discovered that both volume and brightness keys dont work while moving windows. I have tried this with Youtube in google-chrome-stable and there also volume keys do not appear to function while moving any window.
OS: Fedora Linux 35 (Workstation Edition) x86_64
Host: 20RAS13M00 ThinkPad E14
Kernel: 5.15.16-200.fc35.x86_64
Uptime: 4 hours, 16 mins
Packages: 1905 (rpm)
Shell: zsh 5.8
Resolution: 1920x1080
DE: GNOME 41.3
WM: Mutter
WM Theme: Nordic
Theme: Nordic [GTK2/3]
Icons: Papirus-Dark [GTK2/3]
Terminal: gnome-terminal
CPU: Intel i5-10210U (8) @ 4.200GHz
GPU: Intel CometLake-U GT2 [UHD Graphics]
Memory: 2154MiB / 7745MiB
submitted by hackintoshuser to gnome [link] [comments]

2022.01.25 04:31 Merthin1 Possible Amazon Third Party Seller Scam?

I recently purchased something from an Amazon seller that seems a bit fishy - maybe someone here with more knowledge can help shed some light on it. It wasn't a big purchase (<$50).
The item was initially ordered with a shipping date 3 weeks out. I let a week go by and then contacted the seller, letting them know that I wanted to cancel the order since it was going to take too long. They then told me that the item had already been shipped and I would have to return it for a refund. During this time, Amazon's tracker showed that the item hadn't ship yet. Somehow, the correct item arrived the next day but in an Amazon delivery truck. I then requested a return process for a refund through the seller and let a few days pass. I check Amazon again and it showed the item being shipped by FedEx with a provided tracking #. Another few days pass and the FedEx website states that the item was delivered with an unrecognized signature and no package at my address. Amazon's tracker still shows the package as being shipped. The seller supposedly started the return process and provided a UPS return label but I can't see any of this through Amazon's site.
I'm not sure if all of this is just normal since I rarely buy from Amazon seller, but it is an odd series of events. In any case, appreciate any thoughts anyone might have on the situation. Specifically, is it safe to use the UPS return label to send the product back to the seller for a refund?
submitted by Merthin1 to Scams [link] [comments]

2022.01.25 04:31 Epix1235 Screw half cash

Screw half cash submitted by Epix1235 to btd6 [link] [comments]

2022.01.25 04:31 Robinjo1985 Cardi B awarded nearly £1 million in damages after winning defamation case against YouTuber Tasha K | Ents

Cardi B awarded nearly £1 million in damages after winning defamation case against YouTuber Tasha K | Ents submitted by Robinjo1985 to celebritynewsgossip [link] [comments]

2022.01.25 04:31 wowzeemissjane Ground cover with small flowers Australia

Ground cover with small flowers Australia submitted by wowzeemissjane to whatsthisplant [link] [comments]

2022.01.25 04:31 FruitdudeID Pls explain this UI interaction to me
the sound gets play multiple times when i enter the mouse for the first time and then the two buttons and the slider also somehow trigger the "mouse_entered" signal of my root node for this scene. so why does the signal also connect all the child signals ?
for the sound playing multiple times when i enter the mouse is when i set a margin to the container. is this normal ? why are there suddenly 2 boxes when there should only be one with a margin ?
submitted by FruitdudeID to godot [link] [comments]

2022.01.25 04:31 __Snor1aX__ [H] Falchion Knife | Slaughter FN, Hand Wraps | Arboreal BS [W] Bowie Knife | Marble Fade FN

0.02 fv Falchion,+20$ on
b/o Bowie Knife | Marble Fade FN + YOUR ADDS
submitted by __Snor1aX__ to GlobalOffensiveTrade [link] [comments]

2022.01.25 04:31 107090 Donkey Kong rap, but it's Deacon Blues by Steely Dan

Donkey Kong rap, but it's Deacon Blues by Steely Dan submitted by 107090 to DeepIntoYouTube [link] [comments]

2022.01.25 04:31 Lonely_Helicopter918 Why do I see most of rich man or inteligent man balled or having very less hair ?

submitted by Lonely_Helicopter918 to biology [link] [comments]

2022.01.25 04:31 clint1reid How do you feel about this setup?

View Poll
submitted by clint1reid to ArkInvestorsClub [link] [comments]

2022.01.25 04:31 lightmare69 Ive got even more names lol

Ive got even more names lol submitted by lightmare69 to shitposting [link] [comments]

2022.01.25 04:31 Dj_Chubbs28 What's something you wish you could forget?

submitted by Dj_Chubbs28 to AskReddit [link] [comments]

2022.01.25 04:31 Schlongstorm Crossover/Fusion Fics that treat supporting characters like Trailblazer

I've spent the last three months catching up with Trailblazer, a Gundam altpower fic. It may in fact be the single best Worm fanfic, in my opinion. One of the things it does which I think may be unique is how it deepens the fusion elements of the world by incorporating characters from the altpower source. The way that the author finds narratively and thematically useful ways to include them in the Worm universe in a way that both reflects their positions and roles in canon, allows for consistent characterization between the two very different continuities, and does so without feeling like we're being introduced to fifty different OCs every few chapters is absolutely amazing.
There's a lot of different media that's been crossed over with Worm, but in my experience most fics (including my own) either only take the altpower and discard pretty much everything else from the setting, or they incorporate some very explicit multiverse/metaphysical elements to set up the crossover. Trailblazer's elegant construction of the world around Taylor and the people both from Worm canon and various Gundam properties is inspiring and intimidating as an author.
What I'm looking for is any fics that do that even half as well as Trailblazer.
submitted by Schlongstorm to WormFanfic [link] [comments]

2022.01.25 04:31 JBruceLee [Homemade] tried my hand at making tuna sashimi

submitted by JBruceLee to food [link] [comments]

2022.01.25 04:31 Nemo-504 Light reshops tonight for my 3rd shift people out there 💪 Nemo504

submitted by Nemo-504 to walmart [link] [comments]

2022.01.25 04:31 c1rinq What the actual fuck, people are defending this child rapist pedophile by saying it's trans and was stressed out so raped a fucking toddler... What the fuck is wrong with people

submitted by c1rinq to awfuleverything [link] [comments]

2022.01.25 04:31 SirRoderic Is black clover worth watching?

1, Is it funny?
2, Will it make me cry?
3, Is there lewd jokes and or fanservice?
(If the answer to questions 2 and 3 are No, then I'll watch it)
submitted by SirRoderic to Animesuggest [link] [comments]

2022.01.25 04:31 jinnaikousaka $200 bonus from SocialGood for shopping $30+

Previous campaign extended to January 31, 2022 with even higher bonus of $200. It was $100 until Christmas.
Enjoy a $200 bonus in SG token from SocialGood app, which lets you earn cashback in their SocialGood crypto for shopping online from stores like eBay, AliExpress and BestBuy. This is a great time to join because they're offering high promotional cashback rates from some stores - 50% from eBay and 100% from Aliexpress, for example. I'm guessing that these rates won't stay too long and will soon drop down to their default rates of 4-6% across all stores (there are 1800+ stores to shop from).
To get the $200 bonus,

  1. Sign up using the following link:
  2. Make sure invite code 2GCU6A is entered
  3. Within 30 days, go to the “Shopping” tab in your SocialGood app and spend $30 or more shopping, excluding shipping and tax
  4. Be patient and wait for about a month or two until they fully confirm you purchased your items and didn't return them
Once your bonus and cashback reward gets posted as "approved", you can choose to withdraw your earned SG token to a crypto exchange to cash out to your native currency following this guide (see this post also), or simply to hold the token for future value appreciation. They say the more users come onboard, the higher the value of the token will be.
If you sign up with the above link, I get a bonus, too, so thank you in advance!
($200 bonus amount is a limited-time offer and expires on January 31, 2022 - see this page)
Reference images:
submitted by jinnaikousaka to clicksforbeermoney [link] [comments]